Journal: Communications Biology

Article Title: Wnt/β-catenin signaling regulates amino acid metabolism through the suppression of CEBPA and FOXA1 in liver cancer cells

doi: 10.1038/s42003-024-06202-9

Figure Lengend Snippet: a Metabolite analysis using HepG2 cells treated with control, β-catenin, or TCF7L2 siRNA. Each group was analyzed in triplicate. b , c Quantitative analysis of the indicated metabolites using mass spectrometry in the HepG2 cells treated with control, β-catenin, or TCF7L2 siRNA. The y-axis represents the concentration (pmol/10 6 cells) of metabolites. b Levels of arginine and histidine. c Levels of lactate and acetyl-CoA. d Metabolic pathway analysis using metabolites that were commonly altered by the knockdown of β-catenin and TCF7L2. FDR q -value < 0.01 was considered significant. e A simplified schematic representation of the urea cycle and related enzymes. ASS1 , argininosuccinate synthetase1; ASL , argininosuccinate lyase; OTC , ornithine transcarbamylase. The microarray data (Fig. ) were used to generate altered expression values of OTC , ARG1 , ASS1 , and ASL (fold change) in the cells treated with β-catenin (siβ-9 and siβ-10) or TCF7L2 (siTCF) siRNA compared to control siRNA. Unless specified otherwise, data are represented as the mean ± SD of three independent cultures. Statistical significance was determined by Dunnett’s test. * p < 0.05, ** p < 0.01 vs siCtrl.

Article Snippet: Metabolome analysis was conducted according to the C-SCOPE package (Human Metabolome Technologies), using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) for cation analysis and CE-tandem mass spectrometry (CE-MS/MS) for anion analysis based on the methods described previously , .

Techniques: Mass Spectrometry, Concentration Assay, Microarray, Expressing